Widefield

Leica and Andor's DSD2

   A leica DMI6000 INverted microscope is in room 53. It can be used as a widefield system or with the DSD2 spinning disk from Andor.

 

  The Leica widefield fluorescence microscope is identical to the upright fluorescence microscope that forms part of the laser microdissection system. Therefore, it can be is routinely used for widefield fluorescence imaging of large (normally brain slices) samples. The acquisition of large samples is performed by sequential imaging of several fields of view that are then stitched. Stitching is normally performed by the LAS X software, although other approaches exist (e.g. FIJI).